Proteomics is the large-scale study of proteins, particularly their identities, structures and functions, instrumental in both drug and biomarker discovery.
HFL provides a range of analytical services to support proteomic investigations including its BioMSä service for protein quantification. The BioMSä platform is designed for the analysis of multiple analytes in a single sample. By targeting different diagnostic peptides, quantitative data can be obtained from multiple proteins in a single analysis.
Protein separation
Proteomics relies on the ability to separate complex protein mixtures so that
individual proteins can be analysed more easily. HFL is equipped with 2-D
electrophoresis equipment such as the Amersham Biosciences IPGphor and
Multiphor systems, ideal for bottom-up proteomics.
Protein Identification
A Sciex 4000 Qtrap coupled to an Ultimate 3000 nanoflow HPLC is HFL's system of
choice for protein characterisation. Database comparisons with known protein
sequences can be made, or de novo sequencing is also available. HFL also has an
atmospheric pressure MALDI source connected to an LCQ ion trap enabling
alternative MS/MS analysis.
Protein Quantification
Various chemical tagging techniques, such as isotope-coded affinity tags
(ICATs), are now available for protein quantification by mass spectrometry.
Protein Modification Post-translational modifications (PTMs) of proteins
including phosporylation and glycosylation are commonly found while working
with biomarkers. The Sciex 4000 Qtrap gives the capability for single
experiment identification and site determination of PTMs using highly selective
precursor ion scans with high sensitivity MS/MS scans.
Complementary techniques
Immunoaffinity chromatography, western blotting, PCR, receptor based
technology, biosensors and lateral flow technology are just some of the other
techniques available at HFL to complement mass spectrometric analysis.
Collaborations
HFL also has strong links with leading academic institutes, where access to
further high-tech equipment and software such as SELDI, MALDI-TOF, NMR, RT-PCR
and ANN's is always available.
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